Abstract

The objective of this research was to determine the effect of fiber architecture on the cell viability of the electrospun nanofiber (ENF) membrane. Three different ENF membranes (consists of single layer, four layers and six layers of cross-direction ENF layer) were developed and deposited on one side of the acrylic mold. The fibrous side of the mold was placed on the top of a well plate. Mouse osteoblast cell adhesion and proliferation on each of the different kinds of membranes were observed using a fluorescent microscope technique. Results shows that the cell adhered and proliferated on each kind of ENF fiber membranes. Tests shows that the designed ENF membrane has no adverse effect on osteoblast cells, fiber attached to the fiber, and strongly dependent on the fiber layer numbers in the membrane.

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