Effect of extraction solvent on in vitro anti-inflammatory, antioxidant activity, total phenol and flavonoid contents in Codonopsis lanceolata

Abstract

Many plant products from medicinal herbs are historically important for the treatment and prevention of diseases. Medicinal plants are showing a wide range of activities like antioxidant, anti-inflammatory, and anti-aging. This study was investigated to evaluate the phenolic content, DPPH, ABTS radical scavenging rate, and anti-inflammatory activity from Codonopsis lanceolata (CL) root extract. Total polyphenol and flavonoid content showed the highest amount in 30% EtOH extract of CL and followed by 90 °C hot water extract. The DPPH, ABTS radical scavenging activity was also found that the increase was proportional to the concentration, the highest amount in 30% EtOH extract. Cell viability of RAW 264.7 cell for CL extracts was not significantly changed by extracts up to a concentration of 200 µg/mL, and it was confirmed that the macrophage cell showed little toxicity. LPS-treated experimental group significantly inhibited NO production. The effect of extracts of CL against inflammatory mediators (TNF-α, IL-6, and IL-1β) production showed that the anti-inflammatory activity was most excellent in 30% EtOH. These results suggested that CL extracts tested here have potential anti-inflammatory and antioxidant activity, and these effects are differences depending on the extraction solvent.