Abstract

The study aimed to know the effect of commercial vitamin B12 and vitamin B12 extracted from Lactobacillus rhamnosus after testing its ability to produce the vitamin, purifying it in the laboratory and determining the optimal condition for its. The results showed effect vitamin B12 on hematological, physiological parameters in experimental animals, it's have a negative effect on hemoglobin concentration, as it decreased to (11.9) µg/ml, with a significant difference from the control treatment (14.6) µg/ml. The results also indicated that the treatment of laboratory animals with B12 extract at a concentration of ( 2, 4) µg/ml positive effect by a significant increase in hemoglobin concentration rate to (13.48,15.2) g/100ml, respectively, compared with the control treatment. The results of the current study of PLT showed a decrease in the rate of blood platelets to (325.3) 103/µL infected with anemia compared to the control treatment, as it was (346) 103/µL. When the animals were injected with vitamin B12 extract, the PLT increased to (334.5) (387.5) 103/µL for the two concentrations (2,4) µg/ml respectively. In addition, the platelet rate increased when treated with commercial B12 for the two concentrations (4,2) respectively (331.2, 334.7) 103/µL compared with the control and extracted B12 treatments

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