Effect of Exercise on Oxidant and Antioxidant Systems in Rat Kidney Tissue with Hyperthyroidism

Hülya Üstündağ ,Erman Şentürk ,Serkan Yildirim ,Elvin Aliyev ,Ahmet Yildirim

doi:10.7176/jmpb/70-05

Abstract

Oxidative stress is involved in the pathogenesis of many diseases. Hyperthyroidism is associated with increased oxidative tissue damage. The aim of this study is to investigate whether if there is any protective effect of a regular endurance exercise on oxidative stress in kidney tissues of rats with experimental hyperthyroidism. Twenty-three male Spraque Dawley rats were divided into four groups: Control, hyperthyroid, exercise and, hyperthyroid with exercise. Hyperthyroid was induced in rats by subcutaneous injections at a dosage of 250 µg L-thyroxine per kg body weight and hyperthyroidism was confirmed by the measurements of TSH, FT 3 and FT 4 in serum. Endurance training lasted 8 weeks and consisted of 45 minutes daily treadmill running at a speed of 23 m/minute for 5 days a week. The levels of MDA, GSH and, GSH-Px activity were measured in kidney homogenates. MDA level of the hyperthyroid group was significantly higher than those of the control group(p=0.022). On the other hand, the MDA level of the exercise group was significantly lower than the MDA level of the hyperthyroid group (p=0,001). GSH level and GSH-Px activities were lower in the hyperthyroid group comparing the control group (p<0.05). GSH level and GSH-Px activity in the exercise group were higher than compared with the hyperthyroid group (p<0.05). The results of our study show that L-thyroxine injection may increase oxidative stress by causing lipid peroxidation at kidney tissues and endurance training may decrease oxidative stress. Keywords: Endurance training, glutathione, glutathione peroxidase, hyperthyroidism, kidney tissue, malondialdehyde DOI: 10.7176/JMPB/70-05 Publication date: April 30 th 2021

Highlights

Thyroid gland is one of the largest endocrine glands in the body, which secretes thyroxine and triiodothyronine hormones called T4 and T3
In this study, the effect of 8 weeks of endurance exercise on kidney tissue oxidant and antioxidant systems was examined in rats in which experimental hyperthyroidism was created with L-thyroxine
In a study comparing the levels of erythrocyte antioxidant enzyme levels and serum MDA levels with data from the healthy control group of hyperthyroidism patients before and after treatment, MDA levels (p

Summary

Introduction

Thyroid gland is one of the largest endocrine glands in the body, which secretes thyroxine and triiodothyronine hormones called T4 and T3. The most common effect of thyroid hormones in many mammal species is that tissues increase the metabolic rate and rate of oxygen use [1,2,3]. Thyroid hormones show this effect on energy metabolism by making many changes in the activity and number of some mitochondrial respiratory chain components including oxygen consumption and oxidative phosphorylation. Thyroid hormones increase the oxidative system by inducing basal metabolic rate and specific mitochondrial enzymes, resulting in free radical formation [5]. Hyperthyroidism, on the other hand, increases these effects [3]

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