Abstract
Objective To investigate the effect of evodiamine on the proliferation of fibroblasts, synthesis of collagen, and gap junction protein connexin 43 (Cx43) in keloid. Methods The cultured in vitro fibroblasts from patients with keloid were harvested and divided into a control group and evodiamine-treated (0.5, 1.0, 2.0, and 4.0 μmol/L) groups. After treatment for 24, and 48 h, cell activity was determined by methyl thiazol tetrazolium (MTT). The expression of gap junction protein Cx43, and systhesis of collagen type Ⅰ and Ⅲ in vitro after treatment were measured by enzyme linked immunosorbent assay (ELISA). Results At 24 h cell inhibition rate was 6.2%, 9.1%, 12.5%, and 17.6% in 0.5, 1.0, 2.0, 4.0 μmol/L evodiamine-treated groups, respectively. Evodiamine could significantly inhibit the growth of fibroblasts in vitro in a concentration- and time-dependent manner. ELISA results revealed that Cx43 protein absorbance (A) values were 0.252, 0.315, 0.383 and 0.547, those of collagen type Ⅰ were 0.507, 0.412, 0.336 and 0.226, and those of Collagen type Ⅲ were 0.246, 0.228, 0.179 and 0.115 in the 0.5, 1.0, 2.0, 4.0 μmol/L evodiamine-treated experimental groups, respectively. The expression of gap junction protein Cx43 was up-regulated, and the systhesis of collagen type Ⅰ and Ⅲ in vitro was decreased after evodiamine treatment. Conclusion Evodiamine can effectively promote the production of gap junction protein Cx43, increase the level of gap junction intercellular communication (GJIC), inhibit the growth of fibroblasts and the production of collagen type Ⅰ and Ⅲ of keloid in vitro. Key words: Evodiamine; Keloid; Fibroblast; Gap junction intercellular communication; Gap junction protein; Collagen type Ⅰ; Collagen type Ⅲ
Published Version
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