Abstract
The effect of epinephrine on parathyroid hormone (PTH) secretion rate and the molecular nature of immunoreactivity in parathyroid venous blood was assessed in seven anesthetized calves. PTH secretion rate was measured by a technique which involved RIA of parathyroid venous blood that was collected during timed intervals and measured volumetrically. The molecular nature of immunoreactivity in parathyroid venous plasma was evaluated by gel column chromatography and RIAs which recognized specific regions of the PTH molecule, i.e. the amino-terminus or carboxyl-terminus. Plasma calcium concentration was measured by chelometric titration. Epinephrine HCI was infused iv into a normocalcemic calf at rates of 0.025, 0.05, 0.10, 0.25, and 0.40 μg/kg-min. PTH secretion rate progressively increased in response to increased rates of infusion until a near maximal secretion rate was attained at an infusion rate of 0.25 μg/kg-min. Epinephrine HCI was infused iv into five calves at a rate of 0.25 μg/kg-min during and aft...
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