Abstract
The purpose of this study was to predict the optimal storage conditions for inhibiting aflatoxin production in upland rice seeds inoculated with Aspergillus flavus. The effects of storage conditions including moisture (A), temperature (B) and incubation time (C) on aflatoxin (AFG1, AFG2, AFB1, AFB2 and AFTOT) production in upland rice seed after inoculation of A. flavus were studied by response surface methodology (RSM) and Box-Behnken Design (BBD). The concentration range of AFG1, AFG2, AFB2, AFB1 and AFTOT were 0.031–9.078 μg/kg, 0–2.766 μg/kg, 0.021–3.478 μg/kg, 1.145–43.433 μg/kg and 1.197–58.755 μg/kg, respectively. Analysis of model variance indicated that moisture and incubation time (AC) exhibited an extremely significant interaction effect on AFG1, AFB2, AFB1 and AFTOT (p < 0.0001). Multiple regression analysis showed that AFG1, AFG2, AFB1, and AFTOT were the least under the incubation temperature of 35 °C or 15 °C, incubation time less than 15 d, and moisture less than 10% or 10.2%, respectively. After best point prediction and validation, the minimum total aflatoxin (AFTOT) concentration was close to 0 μg/kg under the predicted conditions (A = 35 °C, B = 15 days, C = 10%).
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