EFFECT OF ENTERAL NUTRITION IN COMBINATION WITH L-ARGININE AND GLUTAMIC ACID ON NUTRITIONAL STATUS IN EXPERIMENT

Abstract

The aim of the study is to evaluate the scheme of complex therapy based on enteral nutrition and L-arginine, glutamic acid on the indicators of protein, carbohydrate, and lipid metabolism in the blood of rats with experimental wound defects of the skin under conditions of partial food deprivation.
 Materials and methods. A biochemical study of blood was carried out in 24 sexually mature male rats, which were partially food deprived for 10 days. On the 10th day, a wound was made and the animals were divided into 4 groups: the 1st group of control animals, which received food according to physiological needs; the 2nd group, which continued to be partially food deprived; and the 3rd group of rats, which after being exposed to dystrophy modelling for 10 days, were given compensatory enteral nutrition; the 4th group, which after the food deprivation, received L-arginine and glutamic acid in a dose of 50 mg/kg in addition to enteral nutrition. Blood sampling was carried out by removing the animals from the experiment on the 28th day. The following biochemical indicators were studied: the content of glucose, total protein, albumins, urea, the level of non-esterified fatty acids (NFA), ketone bodies, ammonia and TBC-reactants.
 Results. Comparative analysis of glucose content showed that integrative therapy prevented the development of hypoglycemia under the conditions of partial food derivation (р˂0.05). The positive effect of the combined action of enteral nutrition and L-arginine, glutamic acid on the serum content of total protein was found to be higher by 18% (р˂0.05), and albumin by 39% (р˂0.05), urea and ammonia was by 50% and 40.1% smaller (р˂0.05), NEJK was lower by 32.4% (р˂0.05), the content of ketone bodies was lower by 73.2% (р˂0.05), and TBK- reactants was lower by 50.4% (р˂0.05), compared to the untreated animals, and these indicators did not statistically significantly differ from the control values.
 Conclusion. The integrated therapy based on enteral nutrition and L-arginine, glutamic acid under the partial food deprivation effectively normalized protein metabolism, indicators of lipid and nitrogen metabolism in the body of rats (p˂0.05), relative to untreated animals.