Effect of edible bird’s nest on hemogram and secondary influenza virus infection of mice with aplastic ane-mia

Abstract

Objective To study of the protective effect of edible bird’s nest on aplastic anemia (AA) following secondary infection of influenza virus. Methods Pharmacology of edible bird’s nest was determined by in vitro and in vivo test. The hemagglutination inhibition test and the neutralization test for infected cells were undertaken in vitro, while the AA mice animal model and virus infection model were adopted in vivo. By in vitro and in vivo experiments, the bird’s nest hydrolysates effect on AA mouse blood was observed, in order to determine the bioavailability of edible bird’s nest. Results The hemagglutination inhibition level mouse effect of edible bird’s nest zymolyte N1-Pan and N2-Pan after trypsin hydrolysis were 4 μg/mL and 125 μg/mL, which had relatively high activity of inhibition to hemagglutination. N1-Pan had strong neutralizing capacity against influenza virus (IC50=40 μg/mL) . In animal feeding experiments, the hemogram of AA mice on the 20th day after feeding N1-Pan for 10 days was obviously better than that on the 10th day (improving 10% to 20%) , of which the difference had statistical significance (Fisher’s exact test, P<0.05) . AA mice with only influenza virus inoculation mouse on the 20 th day that N1-Pan for 10 days only after feeding N1-Pan had improved peripheral hemogram and weight the than AA mice with influenza virus inoculation (Fisher’s exact test, P<0.05) , and the survival rate of the former was much higher than the latter (4/8 vs 7/8, Fisher’s exact test, P<0.05) . Conclusions N1-Pan can significantly improve the AA mice peripheral hemogram and body weight, and increase survival rate of AA mice infected with influenza virus. Key words: Anemia aplastic; Edible bird’s nest; Influenza virus; Infection