Abstract
In this study, two cell types from porcine females, namely fetal fibroblasts (pFFs) and adult ear fibroblasts (pAEFs) and two passages (3-4 and 7-8) were investigated by evaluating the development rate, blastocyst cell number and the incidence of apoptosis. No significant differences were observed in the cleavage rates of cloned and IVF embryos. The blastocyst rates between the embryos cloned with pFFs (15.1±3.2) and pAEFs (10.4±2.6) did not differ significantly but was significantly (p<0.05) lower in pAEFs than that in IVF (22.5±4.5) embryos. Total cell number in pFFs (28.4±4.3) and pAEFs cloned blastocysts (24.2±5.1) was significantly (p<0.05) lesser than IVF control (35.4±3.2). Apoptosis rates between cloned blastocysts differed significantly (p<0.05) and were significantly (p<0.05) higher than IVF embryos. The blastocyst rates between the cloned embryos cloned with different cell passages did not differ significantly but in embryos cloned with 7-8 cell passage was significantly (p<0.05) lower than the IVF control. Apoptosis signals were detected in IVF and cloned embryos as early as day 3 and the rates of apoptosis increased concurrently with the embryo development. In conclusion, high apoptosis during in vitro preimplantation development resulted in low development rate and total cell number of cloned embryos. Moreover, based on the apoptotic incidence in cloned blastocysts, fetal fibroblasts are more suitable for production of cloned embryos in porcine.
Highlights
Since the birth of a cloned sheep (Wilmut et al, 1997), somatic cell nuclear transfer (SCNT) in mammalian species has been developed and advanced in the last 8 years
Development of SCNT embryos produced from different donor cell types
No significant differences were observed in the cleavage rates of cloned and in vitro fertilization (IVF) embryos
Summary
Since the birth of a cloned sheep (Wilmut et al, 1997), somatic cell nuclear transfer (SCNT) in mammalian species has been developed and advanced in the last 8 years. During in vitro culture in pigs, the SCNT embryos exhibited higher rates of cytoplasmic fragmentation and developmental arrest as well as high levels of apoptotic cells than IVF embryos (Hao et al, 2003). Apoptosis levels are important parameter and emerging as a useful indicator of early embryonic development and quality With this in view, two cell types, namely fetal fibroblasts (pFFs) and adult ear fibroblasts (pAEFs) and two passages (3-4 and 7-8) were investigated by evaluating the development rate, blastocyst cell number and the incidence of apoptosis in cloned embryos compared to those of in vitro fertilization (IVF) counterparts in porcine
Published Version (Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have