Abstract
Summary The capacity of murine C-1300 neuroblastoma cells to repair x-ray induced DNA single strand breaks was compared under two distinct culture conditions. Growing undifferentiated cells maintained in normal medium containing 10% fetal calf serum showed substantial break rejoining during a 30 minute repair period. Non-growing, differentiated cells in serum-free medium did not appear to be capable of repairing such breaks. Whether this phenomenon is attributable to a loss of repair enzymes or to an increased lability of the differentiated cells related to adverse culture conditions is uncertain. The results suggest that (a) nuclear integrity is not absolutely vital to morphogenetic differentiation of C-1300 neuroblasts and (b) also imply that deterioration of tissue culture growth conditions may affect DNA repair mechanisms in a fashion independent of other complex biosynthetic events.
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