Abstract
The potentials of Leucaena leucocephala leaf-meal as a supplementary protein in fish feed as evaluated. Leucaena leaves were subjected to two processing methods (sundrying and boiling) to determine the effect of these treatments on proximate composition and anti-nutritional factors. Five samples of Leucaena leaves were analyzed, sundried for 24 hours (SD 1 ), sundried for 48 hours (SD 2 ), sundried for 72 hours (SD 3 ), soaked in boiled water for five minutes at 100°C (SB 5 ) and unprocessed (SU). The tests were carried out in triplicate for each treatment. Data obtained were subjected to one way analysis of variance. Sun-drying (SD 1 , SD 2 , SD 3 ) led to a significant (P≤0.05) increase in crude protein, ash, phosphorus, potassium, magnesium and sodium when compared with the soaked in boiled water (100°C) for 5 minutes (SB 5 ) treatment. The sample soaked in boiled water for 5 minutes (SB 5 ) gave 73, 90, 81, 97 and 36 % reduction in the levels of phytate, cyanide, tannin, saponin and mimosine respectively, which was better than the sundried treatments. Key Words : Leucaena leucocephala , anti-nutritional factors, nutritional composition, supplementary protein, mimosine, fish feed.
Highlights
MATERIALS AND METHODSLeucaena leucocephala is a fast growing multipurpose Sample Collection: Leucaena leaves were collected legume that has been introduced to many tropical and from trees planted at the College of Agriculture and subtropical countries (FACT, 1997)
The use of plant protein sources such as soyabeans and reduced through processing (Monoj and groundnut in fish diet may not be sustainable because Bandyopadhay, 2007)
Leucaena leucocephala is a fast growing multipurpose Sample Collection: Leucaena leaves were collected legume that has been introduced to many tropical and from trees planted at the College of Agriculture and subtropical countries (FACT, 1997)
Summary
Leucaena leucocephala is a fast growing multipurpose Sample Collection: Leucaena leaves were collected legume that has been introduced to many tropical and from trees planted at the College of Agriculture and subtropical countries (FACT, 1997). Determination of moisture content (AOAC, 1990): Crucibles were washed and dried to a constant weight in an air oven at 100°C, these were later removed and cooled in a desiccator and weighed (W1). Two grams of the dried sample was boiled with 300ml of distilled water This was diluted in a standard volumetric flask and filtered through a non-absorbent cotton wool. Five grams of the dried ground sample was weighed into a thimble and transferred into the soxhlet extraction chamber fitted with a condenser and round bottom flask. The contents were cooled, and 10ml of Aqua regia was added to the sample in crucible and filtered into a 25ml volumetric flask to make up to volume with de-ionised water. The means were separated using Duncan’s multiple range test at 95% confidence limit
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