Abstract
In agriculture, phytase is one of the most important monogastric animal sources of nutrient components because it effectively catalyzes the release of phosphate from phytate and phosphorylated compounds. In present work, Aspergillus niger strain (own collection) was used. Various physical and chemical factors have been known to affect the growth and the production of phytase. The effect of carbon and nitrogen sources, temperature and pH for extra cellular phytase production was investigated. Maximal phytase activity of Aspergillus niger was detected in media with 1.0% sucrose as a carbon source. Among the inorganic and organic nitrogen sources, ammonium nitrate in concentration of 0.5% was found to be a favorable nitrogen source for phytase production in Aspergillus niger. Optimum temperature and pH for phytase production by Aspergillus niger were 30°C and 5.5.
Highlights
Parts of the major components of poultry fodder are wheat, soybean meal, corn, which serve as an indispensable source of protein, carbohydrates, calcium and phosphorus
Enzyme production was optimized by using different organic and inorganic nitrogen sources such as ammonium sulfate, ammonium phosphate, ammonium nitrate, potassium nitrate, peptone and yeast extract with concentration of 0.5% incorporated in basal medium replacing ammonium sulfate
Effect of Different Carbon Sources on Phytase Production by Aspergillus niger The results indicated that among the various carbon sources used for phytase production, sucrose is a suitable carbon source for maximal phytase activity of 2.1 U/ml (Figure 1)
Summary
Parts of the major components of poultry fodder are wheat, soybean meal, corn, which serve as an indispensable source of protein, carbohydrates, calcium and phosphorus. Those substances are contained in fodder in the form of complex indigestible conglomerates bound by complex organic compounds-phytates and cannot be. Enrichment of the diet by phytase increases availability of phytate protein, phosphorus, calcium, zinc and copper, improves digestibility and stimulates weight gain [1]-[3]. Present investigation involves studies on the effect of carbon and nitrogen sources, temperature and pH on the production of phytase enzyme by Aspergillus niger in submerged fermentation
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