Abstract
BackgroundConsidering the probability of chemical and enzymatic reactions between matrix metalloproteinases (MMPs) in the dentin structure and their specific inhibitors, the aim of the present study was to evaluate the effect of different concentrations of specific inhibitor of MMPs (galardin) on the shear bond strength of self-adhesive resin cements to dentin.Material and MethodsForty-eight sound human premolars were mounted in self-cured acrylic resin after removal of the enamel on the buccal and lingual surfaces. The dentin surfaces achieved were polished and prepared with 600-grit silicon carbide paper. The samples were divided into 3 groups (n=16) based on the concentration of galardin used (with no galardin, galardin at a high concentration and galardin at a low concentration). In addition, 96 composite resin blocks, measuring 3 mm in height and diameter, were prepared. The composite resin blocks were bonded to the buccal and lingual surface dentin with Rely-X Unicem (RXC) and Speed CEM (SPC) self-adhesive resin cements, respectively, according to manufacturers’ instructions. After 24 hours of storage in distilled water at 37°C, the shear bond strength values were determined in MPa and fracture modes were evaluated under a stereomicroscope. Data were analyzed with two-way ANOVA and post-hoc Bonferroni test (α=0.05).ResultsThe shear bond strength of galardin at high concentration was significantly higher than that in the control group and galardin at a low concentrations (P<0.001). In addition, galardin at a low concentration exhibited higher shear bond strength compared to the control group (P=0.005). Furthermore, higher shear bond strength values were reported with the use of RXC compared to SPC (P<0.001).ConclusionsIrrigation with galardin increased the shear bond strength of self-adhesive resin cements to dentin and this increase had a direct relationship with the concentration of galardin in the solution. Key words:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide, Matrix metalloproteinase inhibitors, Dentin, Extracellular matrix, Luting agents, Dental Bonding.
Highlights
Hydrophobic solvents such as acetone and ethanol are incorporated into dentin adhesives in order to facilitate penetration of monomer into the etched dentin and for direct contact of the resin with collagen fibers
Irrigation with galardin increased the shear bond strength of self-adhesive resin cements to dentin and this increase had a direct relationship with the concentration of galardin in the solution
Two-way ANOVA showed that the effects of different concentrations of galardin (F=32.45, P
Summary
Hydrophobic solvents such as acetone and ethanol are incorporated into dentin adhesives in order to facilitate penetration of monomer into the etched dentin and for direct contact of the resin with collagen fibers. The principal and common mechanism of bonding of resin cements to enamel and dentin involves the use of adhesive systems that improve the micromechanical bond to the etched enamel and form a hybrid layer on the dentin [2,16] This strategy has high technique sensitivity and consists of several steps that result in a decrease in bonding efficacy and hypersensitivity after restoration. The aim of present study was to evaluate the effect of different concentrations of galardin on the shear bond strength of self-adhesive resin cements to dentin. Considering the probability of chemical and enzymatic reactions between matrix metalloproteinases (MMPs) in the dentin structure and their specific inhibitors, the aim of the present study was to evaluate the effect of different concentrations of specific inhibitor of MMPs (galardin) on the shear bond strength of self-adhesive resin cements to dentin. Higher shear bond strength values were reported with the use of RXC compared to SPC (P
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