Effect of different Aggregatibacter actinomycetemcomitans strains on dual-species biofilms formed with Porphyromonas gingivalis or Dialister pneumosintes.

Abstract

There are five evolutionarily divergent clades of Aggregatibacter actinomycetemcomitans, with possible differences in phenotype and virulence potential among strains. This study examined the formation of biofilm by each of 11 distinct strains of A.actinomycetemcomitans, alone or after coculture with two species of oral bacteria (Porphyromonas gingivalis ATCC33277 or Dialister pneumosintes ATCC33048). Confocal laser scanning microscopy (CLSM) and electron microscopy were used to characterize the dual-species biofilms of interest. A reduction in dual-species A.actinomycetemcomitans-P.gingivalis biofilms was observed for A.actinomycetemcomitans RHAA1, suggesting an antagonistic relationship. The amounts of dual-species A.actinomycetemcomitans-D.pneumosintes biofilms were either increased or decreased in some - but not all - strains, indicative of strain-specific phenotypes. The CLSM analyses confirmed the existence of an antagonistic relationship between A.actinomycetemcomitans D7S-1 and P.gingivalis ATCC33277, and a synergistic relationship between A.actinomycetemcomitans D7S-1 and D.pneumosintes ATCC33048. The electron microscopy analyses revealed distinct morphological features of A.actinomycetemcomitans D7S-1 and D.pneumosintes ATCC33048 dual-species biofilms. The results indicate that the relationship between A.actinomycetemcomitans and oral bacteria may vary among strains, which could lead to distinct strain-specific patterns of niche sharing in subgingival microbiota.