Abstract
The potentiating effect of DDC (diethyldithiocarbamate) on dapsone hydroxylamine-mediated methaemoglobin formation was investigated in human erythrocytes in static and rotating in-vitro models. The potentiating effect shown in the static model was retained in the non-rotating two-compartment model. Rotation of the two-compartment model caused the effect to be first lost, and finally reversed as rotation speed increased. Rotation was associated with increased oxygenation of the erythrocytes in this system compared with the static system. Although both DDC and dapsone hydroxylamine individually oxidize haemoglobin by similar redox reactions, these processes are clearly different in velocity. Depending on oxygen availability and speed of motion of the incubation mixture, DDC both potentiates the redox cycling of the hydroxylamine in lower oxygen tensions and directly reverses methaemoglobin formation in higher oxygen levels. It is unlikely that DDC or its parent compound, disulfiram would have any methaemoglobin-promoting effect during dapsone therapy in-vivo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
