Abstract
Abstract RATIONALE: The house dust mite, Dermatophagoides pteronyssinus (DP) is a major source of allergens implicated in the pathogenesis of allergic disorders; they include asthma, allergic rhinitis, and atopic dermatitis. Monocyte chemotactic protein 1 (MCP-1/CCL2)/IL-6/IL-8 plays a pivotal role in mediating the infiltration of various cells into the skin of atopic dermatitis and psoriasis. METHODS: The aim of this study was to investigate the effect of DP extract (DpE) on expression of MCP-1/IL-6/IL-8 mRNA and protein and the signal transduction leading to MCP-1/IL-6/IL-8 expression induced by DpE in the human monocytic cell line, THP-1. The mRNA and protein expression of MCP-1/IL-6/IL-8 was measured by performing RT-PCR and ELISA. RESULTS: DpE-stimulated cells showed elevated MCP-1/IL-6/IL-8 expression in a time and dose-dependent course. MCP-1/IL-6/IL-8 expression induced by DpE is strongly inhibited by PP2, an inhibitor of Src, rottlerin, an inhibitor of protein kinase C δ (PKC δ) PD98059, an inhibitor of MEK and SB202190, an inhibitor of p38 mitogen-activated protein kinase (MAPK). CONCLUSIONS: These results indicate that DP increases MCP-1/IL-6/IL-8 expression and transduces the signal through extracellular signal-regulated kinases (ERK1/2) in human monocytes.
Published Version
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