Abstract
Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT® (BTA) Fastidious Antimicrobial Neutralization (FAN® PLUS) bottles (FA PLUS, FN PLUS, and PF PLUS) were tested after delayed entry times of 24 and 36 h at 20–25 °C (room temperature, RT) prior to loading into the BACT/ALERT® VIRTUO® microbial detection system (VIRTUO). Clinically relevant organisms were inoculated into bottles with 5–84 colony forming units (CFU) per bottle, and human blood (0 to 10 mL), and then loaded into the VIRTUO. When bottles were loaded without delay, a mean time to detection (TTD) of 9.6 h was observed. For delayed bottles, the TTD reported by the VIRTUO was added to the 24-h and 36-h delay times and resulted in average time to results of 32.5 h and 42.5 h, respectively. The FAN PLUS bottles in conjunction with the VIRTUO produced acceptable results when delays up to 24 h at 20–25 °C occur in loading.
Highlights
In 2017, the World Health Assembly (WHA) and World Health Organization (WHO) made sepsis a global health priority that causes approximately six million deaths worldwide each year [1]
This study demonstrates the performance of FAN PLUS bottles in conjunction with the VIRTUO® microbial detection system (VIRTUO) when bottles encounter typical delay times in loading of 24 h and 36 h as reported in some clinical laboratories
The observed faster time to detection (TTD) for the hold times of 24 h and 36 h demonstrate microbial growth occurred at room temperature (RT) prior to loading
Summary
In 2017, the World Health Assembly (WHA) and World Health Organization (WHO) made sepsis a global health priority that causes approximately six million deaths worldwide each year [1]. The WHA resolved to improve the prevention, diagnosis, and management of sepsis. Increased mortality rates correlated with delays in diagnosis of blood stream infections (BSI) and subsequent delays in initiation of effective treatment [2]. Blood culture bottles continue to be the gold standard for isolating the infectious agent from a patient. Blood or sterile body fluids are inoculated directly into culture bottles at the patient’s bedside and transported to a clinical microbiology laboratory without delay. Some hospitals have microbiology labs that are closed or under-staffed during off-shift, weekends, and holidays, or, do not have the facilities or equipment to perform blood cultures in-house.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: European Journal of Clinical Microbiology & Infectious Diseases
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
