Abstract
The presence of endometrial tissue outside of the uterine cavity is named endometriosis and is the most common gynecologic disorder in women. Determining the inhibitory effect of a Deforolimus on angiogenesis in a three-dimensional (3-D) culture of human endometrial stromal cells (hEnCs) in vitro. The important mechanism in the pathogenesis of endometriosis is angiogenesis, and deforolimus has been shown to have anti-angiogenic activity. This was an in vitro study of human endometrial stromal cells in 3-D culture of fibrin matrix. Endometrial stromal cells isolated and placed in a 3-D fibrin matrix culture system for angiogenesis with VEGF and inhibit angiogenesis by deforolimus. Finally these cells analyzed by CD31 antibodies. After 3 weeks, in cells treated with VEGF, endothelial cell branching was observed and rudimentary capillary-like structures formed. In the presence of 5μM of deforolimus, angiogenesis was reduced. The deforolimus were shown to be effective in inhibiting the mechanisms of angiogenesis.
Highlights
Endometriosis is a chronic benign gynecological disorder characterized by presence of endometrial cells throughout the uterus or peripheral organs and tissues
The main cellular event observed during the first week of culture of endometrial stem cells (EnSCs) with vascular endothelial growth factor (VEGF) was formation of sheets of cells in the fibrin matrix (Figure 2(A))
Immunocytochemistry analysis showed that endometrial stromal cells after treated with VEGF for 3weeks express endothelial marker CD31 (Figure 3)
Summary
Endometriosis is a chronic benign gynecological disorder characterized by presence of endometrial cells throughout the uterus or peripheral organs and tissues. Recent progresses in the field of stem cells and evidences for in situ presence of endometrial stem cells or migratory stem cells from bone marrow have opened up a new way toward stem cell-based pathogenesis of endometriosis. Stem cells are being considered as an important tool in cell therapy which is a novel therapeutic method by clinical researchers [5,6]. On this basis, endometrial stem cells (EnSCs) with higher ability for proliferation, differentiation and fast angiogenesis during menstruation alongside with their immune tolerance for embryo during pregnancy have been considered as a valuable source of stem cell [7,8]. Our previous studies demonstrated that EnSCs are exceptionally capable of participating in a phenomenon like angiogenesis in 3-D cultures which is similar to early stages of OPEN ACCESS
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