Effect of Daidzein on Ileum Microflora Biodiversity in Hy-Line Variety Brown Layers

Abstract

Daidzein is always added into poultry feed to make the production performance and immunity of poultry better. In this study, a total of 600 40-week-old Hy-Line variety brown layers were randomized into five groups and fed with a corn-soybean-mixed basal diet supplement with 0, 10, 50, 100, and 500 mg · kg−1 daidzein, respectively. Then, two PCR-based typing methods (RAPD-PCR and ERIC-PCR) were combined to analyze the ileum content and explore the changes of ileum microflora biodiversity. The results of RAPD-PCR and ERIC-PCR showed that bands under 10 mg · kg−1 and 50 mg · kg−1 were the most, and their similarity was the largest. Bands under 500 mg · kg−1 were the least and similarity with other groups was the minimum. Ileum microflora biodiversity under 10 mg · kg−1 or 50 mg · kg−1 was richer than that under 500 mg · kg−1. A corn-soybean-mixed basal diet supplement with 10 mg · kg−1 to 50 mg · kg−1 of daidzein might be beneficial to Hy-Line variety brown layers intestinal bacteria.