Abstract
The biotin requirement of Lipomyces starkeyi IAM 4753 was tested in chemically defined media with variations in the initial pH. The strain exhibited normal growth in a glucose-mineral medium with a low initial pH (-5.5) and biphasic growth at a pH of 6.1. A supplement of biotin to the medium resulted in a rise of the pH to 6.5 at which normal growth occurred. Thus, it was considered that the yeast took up biotin from the medium with a high pH and supported their normal growth. When growing cells obtained from the glucose-mineral medium were analyzed for biotin, approximately 38 to 68 times as much biotin was detected as the amount of biotin which had been acquired from seed culture. Therefore, there was no obstruction in the biochemical sequence of the strain leading to the biosynthesis of biotin, but the strain has an enzyme system which is affected by culture pH.
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