Abstract
Avocado shoots were multiplied in vitro in two culture media of different consistency, double phase and solid medium, at three different irradiance levels: 35, 60 and 85 µmol m −2 s −1 . Effects of culture and environmental conditions in multiplication rate, rooting capacity, hyperhydricity and leaf surface morphology of microcuttings were evaluated. Double phase medium induced hyperhydricity, producing leaf microcuttings with deformed stomata and low crystalline epicuticular waxes; microcuttings also showed reduced rooting capacity. By contrast, solid medium promoted leaf area development on microcuttings and decreased hyperhydricity. Stomatal index was not affected by these treatments but stomatal density was, interacting with the amount of irradiance applied. Increasing irradiance decreased concentration of chlorophyll a and carotenoids in the leaf but did not affect leaf hyperhydricity.
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