Abstract
Human diploid cell cultures were used to study the effects of three variables of tissue culture, type of media, frequency of media change and the degree of confluency, upon the specific activity of three lysosomal enzymes, β-glucosidase, aryl sulfatase, and acid phosphatase. Cells were grown in either Ham's Nutrient F-10 or Minimum Essential Medium (Earle salts) supplemented with 15% fetal calf serum. The medium was either changed daily or left unchanged for the 5 days of the study, and the cells were harvested at 24 h intervals and assayed for each of the enzymes mentioned. The specific activity of β-glucosidase and aryl sulfatase was significantly higher when cells were grown in MEM, when either medium was unchanged and at confluency. These variables had no significant affect on acid phosphatase activity.
Published Version
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