EFFECT OF CU(II)2 (3,5-DIPS)4 (H2 O)3 ON SURVIVAL, AVERAGE LIFE AND HEMATOLOGICAL INDICATORS IN ANIMALS WITH BURNS III DEGREE

Abstract

Taking into account the properties of copper: normalization of the endocrine system, participation in the process of hematopoiesis, participation in the synthesis of collagen and elastin, neutralization of toxins, reduction of inflammatory reactions — we used a number of copper chelates to treat burn injuries under experimental conditions. Before discovering possible healing properties for burns, we quantified the toxicological properties of the substance. For this, the average lethal dose LD50/7LD50/7 which is the dose of a substance at which 50% of animals die within 7 days (after subcutaneous injection of the substance), was calculated by the integration method of H. Behrens in experiments on rats. In the second series, we caused grade III burn lesions on the backs of epilated animals, 30% of the body surface. The organometallic complex Cu(II)2 (3,5-DIPS)4 (H2 O)3 was injected into the animals intraperitoneally 30 min after the burn injury (for the first time at a dose of 50 mg/kg). From day 2, chelate was administered at a dose of 20 mg/kg. The injection was done once every 2 days for 10 days until the scab falls off. According to the experimental results, the Cu(II)2 (3,5-DIPS)4 (H2 O)3 metal complex is a compound with low toxicity. When administered subcutaneously in the dose range from 100 mg/kg to 300 mg/kg, behavioral disturbances and death of animals were not observed within 24 hours. We studied 5 hematological indicators: blood clotting time (BCT), the number of leukocytes, erythrocytes, the level of hemoglobin and platelets. Survival and average life expectancy in the group (burn) were, respectively, 40% and 18.8 days, and in the group burn + metal complex — 60% and 19.2 days, which indicates a positive effect of this metal complex on the body. There was a significant difference in clotting time, leukocyte count and platelet count compared to the control group (p<0.05), which indicates the healing properties of the Cu complex in case of burns.