Effect of column temperature on high-performance liquid chromatographic behaviour of inorganic polyphosphates : I. Isocratic ion-exchange chromatography

Abstract

The isocratic elution conditions, column temperature and eluent concentration, at pH 10 for the analysis of inorganic polyphosphates were optimized to minize analysis time and to maximize resolution. The retention time was described as a function of eluent concentration. The column temperature affected drastically both the retention time and the band broadening of polyphosphates. The enthalpy change associated with the retention was calculated to be positive. A computer-assisted retention prediction system based on these relationships was successfully applied to the optimization of the elution conditions. The graphical presentation of the optimal elution conditions provided a simple technique for obtaining an adequate set data on the column temperature and eluent concentration for the complete separation and rapid analysis of inorganic polyphosphates.