Abstract
Different theories have been proposed for the elution of proteins in reversed-phase high-performance liquid chromatography. To establish the correct elution mechanism, the effects of column length and the concentration of the organic solvent on column efficiency and the elution of high- and low-molecular-weight compounds were examined. It was concluded that protein elution principally involves the same retention process as for low-molecular-weight compounds, although the influence of partition is small under steep gradient conditions. In accordance with this, wide-pore packings in a short column (35 mm) gave excellent separations of proteins and were usable with a wide range of gradients.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
