Abstract
The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0,9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain.Keywords: C. carpio L, Egg yolk, Malondialdehyde, Motiliy, Viability
Highlights
Cyprinus carpio L is classified as a type of fish that has high economic value [1]
EYC 15% has a high percentage of viability spermatozoa and a significant difference with EYC 0% and 5%, whereas there was no significant difference between EYC 10% and EYC 15%
Punten strain was maintained at 96 h during cold storage
Summary
Cyprinus carpio L is classified as a type of fish that has high economic value [1]. One of the main factors needed to increase the cultivation and production of C. carpio L is created as the best quality of parental fish to produce good quality seeds. The process of producing the best quality of parental fish required a long time and high cost. The advantage of sperm storage is can be stored for a long time and can be used when necessary [2]. Sperm storage at low temperatures can cause cold shock [3], which can produce ROS (Reactive Oxygen Species) [4]. Malondyladehid (MDA) is the final result of the lipid peroxidation process, which is toxic to cells. MDA can cause damage and decrease the integrity of the spermatozoa membrane, resulting in decreased sperm quality [5]. The use of Brawijaya University, Malang, 65145 storage media can inhibit sperm movement, stabilize the physiology of sperm, and maintain the viability of sperm [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
