Effect of Coconut Water Concentration on Survival of Bench-Dried Periodontal Ligament Cells.

Ai-Jundi Sh,Nizar Mhaidat,Sanaa Al-Haj Ali

doi:10.5005/jp-journals-10005-1074

Ai-Jundi Sh, Nizar Mhaidat + Show 1 more

Open Access

https://doi.org/10.5005/jp-journals-10005-1074

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Abstract

ABSTRACTBackground : Coconut water is a biological and sterile liquid. It contains a variety of electrolytes, sugars and amino acids. The purpose of this study is to evaluate the effect of concentration and maturity of coconut water on its ability to preserve human PDL cell viability after exposure to dry time of up to 120 minutes using an in vitro cell culture model.Methods : PDL cells were obtained from sound permanent first molars which were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM). Cultures were subjected to 0, 30, 60, 90 and 120 minutes dry periods then incubated with 100 and 50% young and mature coconut water for 45 minutes at room temperature (18-26°C). Untreated cells at 0 and 120 minutes, and cells incubated in DMEM served as controls. PDL cell viability was assessed by MTT assay. Statistical analysis of data was accomplished by using one-way analysis of variance complemented by Tukey test, and the level of significance was 5% (p < 0.05).Results : 100% mature coconut water (MCW) was better than 50% dilutions obtained from mature or young coconuts. However, no significant benefit to the cells was noticed from the addition of the soaking step prior to 30 minutes dry time.Conclusion : Avulsed teeth which are left dry for > 30 minutes may be benefited from soaking in 100% mature coconut water; further studies on simulated avulsion in animal models are needed to verify the above results.

Highlights

Tooth avulsion represents 0.5 to 3% of traumatic injuries to permanent teeth
Coconut water was found to be superior to propolis, milk and Hank’s balanced salt solution (HBSS) in preserving periodontal ligament (PDL) cell viability after exposure to 30 minutes dry time.[8]. These studies neither indicate the concentration of coconut water used nor the maturity of the fruit used to collect coconut water from, in addition the efficacy of coconut water to maintain PDL cell viability after exposure to extended periods of dry time was not tested before, the main aim of the present study is to evaluate the effect of concentration and maturity of coconut water on its ability to preserve human PDL cell viability after exposure to dry time of up to 120 minutes using an in vitro cell culture model
The results of the present study showed that beyond 30 minutes of dry time, PDL ligament cells benefited from soaking in 100% coconut water

Summary

Introduction

Tooth avulsion represents 0.5 to 3% of traumatic injuries to permanent teeth. It is one of the most severe injuries characterized by complete displacement of the tooth outside the socket.[1] Treatment of avulsion usually focuses on encouraging viability and repair of the periodontal ligament (PDL).[2] This is usually achieved by replantation within the first 5 minutes of the event (immediate replantation).[3] this is not always possible under certain conditions. Coconut water is a biological and sterile liquid. It contains a variety of electrolytes, sugars and amino acids. The purpose of this study is to evaluate the effect of concentration and maturity of coconut water on its ability to preserve human PDL cell viability after exposure to dry time of up to 120 minutes using an in vitro cell culture model

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