Human periodontal fibroblasts viability stored in Custodiol® , coconut water, and propolis. An ex vivo study.

Abstract

Successful replantation of an avulsed tooth depends on the regeneration of periodontal ligament (PDL) attachment which is affected by the transport medium, dry time, and storage time. Various storage media have been studied but the search for the optimum storage medium is still needed to determine the ideal material and storage time to maintain PDL cells. The aim of this study was to determine the ability of Custodiol® , coconut water (CW) from different stages of maturity, and propolis as storage media for avulsed teeth by evaluating the viability of PDL cells for different time intervals. PDL cultures were subjected to Cutodiol® , immature, half mature, and mature coconut water, and different concentrations of propolis in Dulbecco's Modified Eagles Medium (DMEM). Culture plates with the tested media were incubated for 1, 2, 6, 24, 48, 72, and 168hour. PDL fibroblast cell viability was assessed by MTT assay. CW showed significantly higher viability of cells than other groups at 6hour with half mature CW being superior. Propolis at 6.25mg/mL in DMEM resulted in 138% viable PDL and it was able to preserve PDL cells for up to 168hour. Half mature and mature CW are superior storage media if replantation of avulsed teeth is within 6hour. Propolis in DMEM could be a potential storage media for prolonged storage intervals up to 48hour.