Abstract
Introduction. Thrombotic complications caused by the tumor and consequences of its treatment are the leading causes of death in cancer patients. The development of a model for the pathology of hemostasis, in particular the excessive pathological clot formation, in the laboratory animals receiving antitumor agents, could help find new pharmacological methods for correcting hemostatic disorders.The purpose of the study was to study the effect of cisplatin on the blood coagulation system in mice and rats.Results. An experiment using outbred mice showed that the levels of PT-INR and aPTT were decreased and the level of fibrinogen was increased on day 10 after administration of cisplatin in the maximum tolerated dose of 10 mg/kg. A significant decrease in the PT-INR and aPTT levels was observed on day 15 after cisplatin injection only in female mice. The cisplatin injection at a dose of 4 mg/kg resulted in a decrease in the PT-INR, and aPTT levels and an increase in fibrinogen concentration on day 10. In rats, a significant decrease in the PT and aPTT levels was observed in both females and males on day 15 after cisplatin injection.Conclusion. A change in the PT and NIR, aPTT levels towards decrease and fibrinogen concentration towards increase indicates the initiation of thrombus formation.
Highlights
Thrombotic complications caused by the tumor and consequences of its treatment are the leading causes of death in cancer patients
An experiment using outbred mice showed that the levels of PT-INR and aPTT were decreased and the level of fibrinogen was increased on day 10 after administration of cisplatin in the maximum tolerated dose of 10 mg/kg
A significant decrease in the PT-INR and aPTT levels was observed on day 15 after cisplatin injection only in female mice
Summary
Целью исследования явилось изучение влияния цисплатина на показатели коагуляционного звена гемостаза экспериментальных мышей и крыс. В эксперименте на аутбредных мышах было показано, что на 10-е сут после введения цисплатина в максимально переносимой дозе (10 мг/кг) наблюдалось снижение показателей активированного парциального тромбопластинового времени (АПТВ, сек), протромбинового времени (ПВ, сек), международного нормализованного отношения (MHO), а также возрастание концентрации фибриногена. На 15-е сут исследования отмечалось значимое уменьшение ПВ, МНО и АПТВ только у самок мышей. Введение цисплатина аутбредным крысам в максимально переносимой дозе 4 мг/кг также вызывало снижение ПВ и МНО, АПТВ и повышение концентрации фибриногена на 10-е сут. У крыс на 15-е сут исследования наблюдалось значимое снижение показателей ПВ и АПТВ как у самок, так и у самцов. Изменение показателей ПВ и МНО, АПТВ в сторону уменьшения, а также увеличение концентрации фибриногена свидетельствуют о запуске процессов тромбообразования.
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