Effect of chronic fish oil supplementation on renal function parameters of mice fed with a high fat or a high carbohydrate diet (689.9)

Abstract

Fish oil (FO) supplementation, rich in n‐3 PUFA, has a positive effect on various diseases, including cancer, kidney diseases and diabetes. High fat (HFD) and/or high carbohydrate (HC) diets promote obesogenic effects and induce insulin resistance.The objective of this study was evaluate the effect of chronic fish oil supplementation on renal function parameters of mice fed with a HFD or a HC diets. Six weeks male mice were divided in three groups and fed with different diets during eight weeks: regular rodent chow (S), HC or HFD. After this, they were supplemented orally for 30 days with FO, 1g/kg body weight. Renal function parameters, urinary excretion of TXB2 and PGE2, immunolocalization of vimentin, desmin, α‐SMA and PCNA, and intraperitoneal glucose tolerance test were measured in all groups. Fasting glucose levels was significant higher in HFD vs S (139.6±6.2 mg/dL vs 104.5±5.5) (n=13).The HFD group present glucose intolerance, an effect that was not modified by fish oil supplementation. Plasma insulin levels were similar between the groups. Glomerular filtration (creatinine clearance) was lower in HC (0.63±0.10ml/min/kg) and HFD (0.78±0.1) groups if compared to S group (1.58±0.4) (p<0.01, n=10). FO supplementation did not induced significant changes in this parameter. Urinary excretion of TXB2 was also significantly lower in HC (2.0±0.4pg/min) and HFD (2.3±0.6) than S (11.3±3.3). The mean microalbuminuria level (expressed as urine albumin‐creatinine ratio) in the C group was 11.06±2.16ng/mg, a value that increased to 18.24±2.74 in HC and 35.65±7.32 in HFD. Fish oil supplementation in HFD partially reverted the increase in microalbumin excretion (21.33±1.33).The intrarenal expression of vimentin was significantly higher in the interstitium of HC and HFD groups, and in HFD tubules, an effect reverted by FO supplementation. In summary, FO supplementation partially reverted renal alterations induced by HC and HFD diets.Grant Funding Source: Supported by CAPES (Brazil)