Effect of chlordiazepoxide hydrochloride on human chromosomes

Abstract

Exposure of human leukocytes in vitro to concentrations of chloridazepoxide hydrochloride of 0.1, 1.0., 10.0, and 100.0 μg per cubic centimeter for varying periods of time failed to demonstrate any increase in incidence of chromosome breakage, gaps, or abnormal forms when compared to control cultures. Analysis of leukocyte cultures from 10 patients taking chlordiazepoxide hydrochloride in varying concentrations were compared to cultures from 6 controls. No increase in chromosome breaks, gaps, or abnormal forms was noted. Studies on the effect of chlordiazepoxide hydrochloride on the growth rate of human fibroblasts in culture showed little variation from control cultures up to concentrations of 20.0μg per cubic centimeter. Decrease in growth rate was noted at concentrations of 50.0 and 100.0 μg per cubic centimeter.