Effect of Chemical Nematicides, Chicken Manure and Biocontrol Agents as a Control Method for Certain Plant Parasitic Nematodes Infecting Orchards under Field Conditions in Sharkia Governorate, Egypt

Abstract

A field treatment was conducted to evaluate the nematicidal activity of chicken manure and biopesticides (bio- arc , bio-zeid and nemex) viz. chemical nematicides (fydal 24 % SL , tervigo (2% SC), laguna (40% EC) and nemathorin (10% G) against plant –parasitic nematodes infecting fruit orchard located in Sharkia Governorate during 2018. Treatments of chicken manure was at the rate of 30 Kg/ tree , while that of biopesticides were applied at rate of 160 g/tree with bio-arc and bio-zeid , whereas nemex was applied at rate of 24 mL/ tree. Chemical nematicides were applied at rates of 15 mL//tree , 50 g/ tree , 24 mL/tree and 12 mL with fydal 24 % SL, tervigo , laguna and nemathorin , respectively. Samples were taken monthly during three months after application. Results indicated that of the eight tested materials, fydal was the most effective in suppressing umbers of phytonematodes followed by chicken manure while among biopesticides , bio- arc was the least effective one. After one month of application, remarkable decreased in numbers of plant-parasitic nematodes was detected. For instance, in fydal and chicken manure treatments, percentages of reduction in numbers of Tylenchulus semipenetrans, Pratylenchus spp., Tylenchorhynchus spp., Hoplolaimus spp. and Helicotylenchus spp. were diminished by 49.75 %(40.92 %) ,56.97% (53.54%), 55.47% (55.83%), 50.53% (50.53%) and 52.52 % (50.42%), respectively. After two months of application, percentage of reduction was increased to reach 69.25 %(68.53%) ,81.26% (71.37%), 83.18% (80.08%), 86.92% (83.00%) and 81.77 % (61.93%), respectively for mentioned nematodes. On the other hand, after three months of application, no significant differences ) P ≤ 0.05) was noticed between fydal and chicken manure whereas, the biopesticides were the least effectiveness against plant –parasitic nematodes. Generally, results emphasized that chicken and biopesticides could be used to minimize the population density of plant- parasitic nematodes in citrus orchards.