Abstract
Stachybotrys atra was found to utilize sucrose and asparagine as the best carbon and nitrogen sources. A temperature of 30°C and pH 5.6 was found to be optimum for its growth. The fungus secretes a dark brown to black thermostable toxic metabolite in the medium. Maximum production of antifungal substance was observed at 26°C and at pH 5.0 using maltose and sodium nitrate as the carbon and nitrogen sources. Importance of the metabolite produced by S. atra is discussed.
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