Effect of cAMP on protein binding activities of three elements in upstream promoter of human CYP11A1 gene

Abstract

CYP11A1 gene encodes the P450scc enzyme, which catalyzes the first step of steroidogenesis under the modulation of cAMP signal. To understand the signaling pathway of cAMP, we analyzed three elements in upstream cAMP-responsive sequences of CYP11A1 gene. The sequence of -1617/-1609 fragment was identical to the conserved SF1 binding site (SF1/Ad4BP). Both sequences of -1637/-1626 and -1559/-1545 fragments are highly homologous to consensus TRE or CRE. The cAMP-treated adrenal Y1 nuclear extracts formed significantly higher intensities of complexes with these three elements than untreated Y1 nuclear extracts. The EMSA demonstrated potential binding of SF1 protein to -1617/-1609, and CREB like protein and AP1 family to -1637/-1626 and -1559/-1545. This is the first report to describe the enhancement of cAMP on protein binding activities of three elements of CYP11A1 promoter to SF1/Ad4BP or CREB/AP1-like proteins.