Abstract
THE cardiac glycoside ouabain binds specifically to the Na pump on cell membranes, so that under defined conditions it can be used to estimate the number of these sites1. When the Na pump of cultured cells is partially blocked by ouabain2 or by ethacrynate (Table 1) the cells respond by increasing their number of pump sites. Our hypothesis for this effect is that the rise in [Na]1 which follows partial blocking of the pumps causes new pumps to be produced or exposed2. The present experiments were started to test this hypothesis in another way, by producing cells with a high [Na]1 by growing them in a low potassium medium. Rather to our surprise we found that this produced a large drop in the number of Na pump sites in these cells. We think this is mainly due to an effect of the dialysed serum in these experiments.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
