Abstract

Influence of 24-epibrassinolide and 28-homobrassinolide on the growth of coleus [Plectranthus forskohlii; Lamiaceae] was investigated. Application of brassinosteroids enhanced the growth in terms of plant height, leaf number, leaf area, and the dry weight of shoots and leaves. Among all the treatments, 28-homobrassinolide at 3μM concentration was highly effective in enhancing the growth. A two-fold increase in shoot dry weight and 2.4 times increase in leaf area was observed in coleus plants treated with 3μM concentration of 28-homobrassinolide. Application of brassinosteroids also increased the rate of photosynthesis, levels of chlorophyll pigments, and various carbohydrate fractions. The tuberous root yield of coleus plants was found to be increased in plants treated with brassinosteroids. 28-homobrassinolide (3μM concentration) proved to be highly efficient and accounted for a twofold increase in tuberous root yield. HPLC analysis revealed the increase in forskolin content in the roots of brassinosteroid-treated plants. Tuberous roots obtained from coleus plants treated with 28-homobrassinolide contained 0.17% forskolin as compared to 0.098% in the roots of untreated control plants.

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