Abstract
Young castrated male goats ( n=22) were used to investigate the effects of administration of a sustained release recombinant bovine growth hormone (GH; 100 mg) at seven-day intervals on body growth, organ weights and lipid content, and on lipogenic responses of cultured explants of omental adipose tissue. Average body weight gain was improved by 22% and the relative weights of the liver, kidney and adrenal glands were increased 16, 19 and 20%, respectively, by GH treatment. The fat content in m. longissimus dorsi and omentum was less in GH-treated animals than in controls by 29.5 and 44.4%, respectively. The rate of fatty acid synthesis was determined in acute incubations both in freshly prepared and chronically cultured omental adipose explants. Adipose explants remained metabolically active and retained their ability to respond to hormones when maintained in tissue culture medium. Cortisol acted synergistically with insulin to produce a higher rate of lipogenesis than that in cultures with insulin alone, but this was the case only in tissues from GH-treated animals; however, cortisol alone decreased lipogenesis in explants from both groups of animals. GH inhibited insulin-stimulated lipogenesis in tissues from control animals and insulin plus cortisol-stimulated lipogenesis in tissues from both control and GH-treated animals. GH treatment in vivo and in vitro did not increase the explant's responsiveness to noradrenaline in vitro; however, responsiveness (inhibition of lipogenesis) to isoprenaline was greater in GH-treated animals than in controls.
Published Version
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