Effect of bone marrow mesenchymal stem cell transplantation on plasma TNF-α level in rat model of emphysema

Abstract

Objective To investigate the effect of bone marrow mesenchymal stem cell (MSCs) transplantation on plasma TNF-α levels in rats of pulmonary emphysema. Methods Thirty wistar rats were randomized into three groupes:normal control group, pulmonary emphysema group, pulmonary emphysema +MSCs transplantation groupe. The pulmonary emphysema model of rats were established by intratracheal instillation of lipopolysaccharide twice and daily exposure to smog. Bone marrow MSCs were infused through tail vein. Morphologic changes of the lung tissues were observed. The level of plasma TNF-α were dectected using enzyme-linked immunosorbent assay. Results Destruction of alveolar walls was observed in rat lungs from pulmonary emphysema group and pulmonary emphysema +MSCs transplantation group. MSCs transplantation group significantly ameliorated the emphysematous changes. Significant differences in the levels of TNF-α between pulmonary emphysema group and pulmonary emphysema +MSCs transplantation group were observed. Conclusions MSCs therapy shows protective effects against pulmonary emphysema. Key words: Bone marrow ; Mesenchymal stem cells ; Pulmonary emphysema ; Cytokine ;