Effect of Bone Marrow Allotransplantation on Neurotransmitter Structures of Lymph Nodes

Abstract

Despite the many factors affecting the state of the lymph nodes, a special role is played by biogenic amines synthesized by the cells of the APUD system and specialized macrophages, which in the context of this study are designated as granular luminescent cells (GLC), as well as mast cells (MC). The presence of receptors for biogenic amines on lymphoid cells proves the subordination of the immune system to neurohumoral effects.The aim of the study was to identify neuroamine-containing cells in the lymph nodes and to determine the content of catecholamines (CA) and serotonin (ST) in them early after bone marrow allotransplantation (ALT).Material and methods. The experiment was carried out on outbred male rats. The 1st group consisted of intact animals (n=10), the 2nd – control individuals, which were injected with 3.0 ml of 0.85% sodium chloride solution (n=10); 3rd – animals (n=30), which underwent bone marrow ALT. To identify neuroamine-containing cells, a luminescence-histochemical study was carried out according to Falk–Hillarp method in Krokhina modification (1969), the proliferative activity of cells was assessed using an immunohistochemical reaction for Ki-67. Statistical analysis was performed using the Statistica-6 program.Research results. After 40 min in the GLC of the corona of the lymph nodes, an increase in the content of CA and ST by a factor of 1.2 was observed; after 2 hours, the CA content increased 1.7 times, and ST – 1.4 times, and after 4 hours there was a sharp decrease in the CA content by 3.6 times. In the GLC of the reactive center and in the reticuloendothelial GLC, the content of both CA and ST decreased. In the GLC of the paracortical zone, the content of CA and ST increased, and after 4 hours their decay occurred. In MC, the content of CA and ST decreased. MC degranulation was observed against the background of a sharp decrease in their number. Due to the degranulation of MC, starting from the 40th min after ALT, neuroamines accumulated in the intercellular space. After ALT, the Ki-67 index changed at different periods of the experiment, in the marginal sinuses it decreased by 13%, in the intermediate sinuses – by 61%, in the lymph nodules – by 47%, and in the paracortical zone – by 40%.Conclusion. Thus, bone marrow allotransplantation leads to a change in the number of granular luminescent and mast cells, as well as the content of biogenic amines in the cervical lymph nodes.