Effect of blood contamination on the fluorescence polarization value of amniotic fluid lamellar bodies.

Abstract

Estimation of fetal lung maturity by examination of amniotic fluid samples is unreliable in the presence of blood. Centrifugation of amniotic fluid at 10,000 X g separates the surfactant-containing lamellar bodies from contaminants, such as serum lipids. The influence of serum contamination of amniotic fluid was evaluated using the fluorescence polarization technique for determination of fetal lung maturity. Addition of serum changed the amniotic fluid P value of both the amniotic fluid and the lamellar body pellets in the direction of the P value of serum. After centrifugation at 10,000 X g, there was no significant difference in the P value of the pre- and postcontamination samples, demonstrating that centrifugation resulted in the removal of pulmonary nonrelevant lipids in contaminated amniotic fluid specimens. In 24 patients in whom the amniotic fluid sample was blood stained, the P value on the lamellar body pellet compared favorably to that obtained from noncontaminated samples.