Effect of Blood Component Coatings of Enosseal Implants on Proliferation and Synthetic Activity of Human Osteoblasts and Cytokine Production of Peripheral Blood Mononuclear Cells.

Lucie Himmlova,Jirina Bartova,Hana Hulejova,Jiri Suttnar,Dana Kubies,Jana Stikarova,Tomas Riedel,Vlasta Pesakova

doi:10.1155/2016/8769347

Abstract

The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a cytokine profile of peripheral blood mononuclear cells (PBMCs) under conditions imitating the situation upon implantation. The cells were cultivated on coated Ti-plasma-sprayed (Ti-PS), Ti-etched (Ti-Etch), Ti-hydroxyapatite (Ti-HA), and ZrO2 surfaces. The plasma/platelets coating supported osteoblast proliferation only on osteoconductive Ti-HA and Ti-Etch whereas activated plasma enhanced proliferation on all surfaces. Differentiation (BAP) and IL-8 production remained unchanged or decreased irrespective of the coating and surface; only the serum and plasma/platelets-coated ZrO2 exhibited higher BAP and IL-8 expression. RANKL production increased on serum and activated plasma coatings. PBMCs produced especially cytokines playing role in inflammatory phase of wound healing, that is, IL-6, GRO-α, GRO, ENA-78, IL-8, GM-CSF, EGF, and MCP-1. Cytokine profiles were comparable for all tested surfaces; only ENA-78, IL-8, GM-CSF, and MCP-1 expression depended on materials and coatings. The activated plasma coating led to uniformed surfaces and represented a favorable treatment especially for bioinert Ti-PS and ZrO2 whereas all coatings had no distinctive effect on bioactive Ti-HA and Ti-Etch.

Highlights

Bone/implant healing process can be enhanced and accelerated by a mechanical/physicochemical [1,2,3] and/or biological treatment of the surface [4]
Ti-PS was placed after TCPS in Table 1 for the following reason: considering that γ is determined by surface properties based on physicochemical interactions on the atomic level, the surface free energy values obtained for the polished titanium in the previous project, γ = 47.01 ± 1.54, γ and its polar (γP) = 15.97 ± 0.54, and γD = 31.05±0.98, could be applied approximately to Ti surfaces with variable roughness values [48]
We intended to evaluate the effect of particular blood components on proliferation and synthetic activity of osteoblasts and cytokine production of human peripheral blood mononuclear cells cultivated on commonly used implant materials, that is, Ti-HA, Ti-Etch, Ti-PS, and ZrO2 ceramic in one complex study

Summary

Introduction

Bone/implant healing process can be enhanced and accelerated by a mechanical/physicochemical [1,2,3] and/or biological treatment of the surface [4]. The biological treatment includes the surface treatment with proteins of extracellular matrix such as fibrinogen/fibrin, collagen and other fibrous proteins, glycoproteins (e.g., fibronectin, laminin), glycosaminoglycans, growth factors (e.g., bone morphogenetic proteins), or specific peptide sequences (e.g., RGDbased sequences). The biological modification includes a treatment with the complex media such as platelet-rich plasma (PRP) [9,10,11,12,13,14] or whole blood [9]. Platelets perform many functions, including formation of a blood clot and release of growth factors (GF) into the wound. The GFs (e.g., platelet derived GFs, transforming GF beta, BMPs, or insulin-like GF) assist the body in repairing itself by stimulating stem cells to regenerate

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Conclusion