Abstract
1. When Piricularia oryzae was grown in the medium containing glucose alone as a carbon source, it was found that the carbohydrate of the mycelium was composed of glucose and its noncellulose polymer.2. Effects of Blasticidin S (BcS) and 2, 4-dinitrophenol on the utilization of glucose by P. oryzae were investigated. The growth-inhibitory concentration of BcS (1.0μg/ml) caused an accumulation of 70 percent ethanol-soluble polysaccharide in the mycelial cells and inhibited completely the synthesis of 70 percent ethanol-insoluble polysaccharide.3. The effect of BcS on glucose utilization was not altered by the addition of L-glutamic acid in the medium. Although the rates of oxygen uptake and glucose utilization by the mycelium treated with BcS were low, the rates were maintained undecreased during 4 hours' incubation in medium. In contrast, the rate of utilization of L-glutamic acid remained unchanged in the first 2 hours' incubation as compared with that by non-treated mycelium, but, after that time, no further utilization was found until 4 hours' incubation.
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