Abstract
Objective: To investigate the influence of bizhongxiao decoction(BZXD) which is a Traditional Chinese medicine for RA including, on the plasma TNF-α and IL-1β in rats with CⅡ-induced arthritis (CIA) and explore the protective mechanism of BZXD in the treatment of rheumatoid arthritis . Methods: 75 SD rats were divided into four groups randomly. Normal control group (n=5) not be treated any more. The CIA rat was established by subcutaneous injection with bovine Ⅱ collagen(BⅡC) and complete Freund,s adjuvant(CFA) after 7d breeding. The CIA rats were divided into the CIA group(n=16)、BZXD group (n=29) treated with BZXD and the MTX group(n=25) treated with methotrexate. All rats were killed after various intervals(25,30,35,40,or 45d). At the end of each time interval, we collected the blood of each rat. To detect TNF-α and IL-1βin plasma with radio-immunity kit. Results: BⅡC and CFA can be used to copying CIA model. The incidence of arthritis was 88%. The plasma TNF-α and IL-1βlevels of CIA group, BZXD group and MTX group were notably higher than those of normal control group(p<0.05), moreover, the CIA group was higher than those of the MTX group and BZXD group at various interval (p<0.01). TNF-α and IL-1βrose step by step in CIA group but decreased in BZXD group and MTX group gradually. Moreover, in BZXD group were lower than those in MTX group (p<0.05). Conclusion: TNF-α and IL-1βplay a very important role in the formation and development of RA. BZXD can notably decrease the plasma TNF-α and IL-1βlevels, which was better than MTX.
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