Abstract
Biotin is a water soluble vitamin which has a well proven function in corboxylation reactions in animal tissues. It is well documented that biotin deficiency in mammals causes pathological changes in the skin and its appendages such as desquamative dermatitis and alopecia. Previous work (Tahmasbi et al 1996) has suggested that deficiency of biotin in the culture medium reduced the viability of wool hair follicles in vitro by mechanisms unknown, but which may involve interference with cell division and or differentiation. The objective of this work was to investigate the effect of biotin deficiency and adequacy on cell proliferation and DNA synthesis as determined by incorporation of [methyl-3H]thymidine in isolated mohair follicles.
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