Abstract
The goal of this study was to analyze the effect of different medium bases on accumulation of biomass and secondary metabolites production by Gardenia jasminoides Ellis. cell suspension culture. The result revealed that different media have significant effect on biomass accumulation and production of polyphenol compounds. HPLC analyses showed that the higher polyphenol content was achieved when the cells were cultivated on McCOWN Woody plant Medium (WP) media for 14 days on darkness, at 24°C. At this conditions, the cells produced maximal amounts of catechin (24.44±2.46 µg/g DW), chlorogenic acid (20.26 ±3.68 µg/g DW), epicatechin (70.44±7.46 µg/g DW) and rutin (45.90±0.26 µg/g DW). Both MS and WP media were optimal for biomass accumulation. When cultivated on these media, Gardenia cells accumulated maximal amount of dry biomass ADB = 0.99±0.1g/100ml and ADB = 0.93±0.04 g/100ml for МS and WP, respectively. The corresponding growth indexes were GIDW = 1.68±0.2 on MS and GIDW = 1.58±0.07 on WP medium. The reported results are the first step of future optimization of the nutrient medium composition and cultivation conditions essential for the scale -up of cultivation process of G. jasminoides cell suspension culture for mass production of polyphenolic compounds.
Highlights
Plants are extremely rich in various chemical compounds that play a crucial role in their growth and adaptation
Cultivation was performed in Erlenmeyer flasks (1L) and a working volume of 200 ml, on a rotary shaker at
The methanol was evaporated under vacuum on 50°C and after that we used solid phase extraction (SPE) – the columns was preconditioned according to manufacturer protocol (Sep-Pak ® Clasic C18 Waters Corporation Milford, Massachusetts USA)
Summary
Plants are extremely rich in various chemical compounds that play a crucial role in their growth and adaptation These compounds are called secondary metabolites, some of which are phenols, terpenes, alkaloids and steroids. An important point of working with in vitro cultures is to optimize the growing conditions so that for a short growth cycle to produce the optimal amount of biomass and secondary metabolites that are of crucial importance to the industry. This is related to a number of studies on the type of plant, the potential of the culture, different nutrient options, and the monitoring of biomass growth and metabolites accumulated in it
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