Effect of BAP and IBA on shoot regeneration of strawberry (&lt;em&gt;Fragaria x ananassa&lt;/em&gt; Duch.) through runner tip culture

H K C Madumali,P D Abeythilakarathna,T H Seran

doi:10.4038/sljfa.v5i1.69

Effect of BAP and IBA on shoot regeneration of strawberry (<em>Fragaria x ananassa</em> Duch.) through runner tip culture

H K C Madumali, P D Abeythilakarathna + Show 1 more

Open Access

https://doi.org/10.4038/sljfa.v5i1.69

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Abstract

Effect of three concentrations (0, 0.5 and 1.0 mg/L) of 6-benzylaminopurine (BAP) in combination with three concentrations (0, 0.5 and 1.0 mg/L) of Indole-3-butyric acid (IBA) on shoot regeneration of strawberry runner tips was investigated. Runner tips were rinsed with water containing Teepol™ for 5 min., kept under running tap water for 30 min, and immersed in 0.5% Topsin™ for 30 min before sterilizing with 20% sodium hypochlorite solution (Clorox™) for 12 min. followed by 70% Ethyl alcohol for 1 min. After sterilization, explants were trimmed and tips of runners (1.0-1.5 cm long) were isolated and cultured on MS media supplemented with different concentrations of BAP and IBA. Among nine treatments, highest number of shoot buds were observed in 0.5 mg/L BAP alone medium followed by 0.5 mg/L BAP + 0.5 mg/L IBA supplemented medium. Highest number of leaves was also observed at the same concentrations. Among nine BAP and IBA concentrations, highest shoot and root length were observed at hormone free medium. Highest shoot girth was observed at 0.5 mg/L BAP + 0 mg/L IBA supplemented medium. It can be concluded that MS basal medium supplemented with 0.5 mg/L BAP could be used to regenerate shoots from runner tips for plant propagation of strawberry.

Highlights

Strawberry is an introduced horticultural crop in Sri Lanka and one of the best soft fruits in many parts of the world
Runner tips of strawberry were used for the establishment of in vitro culture
The results of the present study proved that the high concentration of cytokinin reduced the number of shoots, and that 0.5 mg/L BAP is the optimum concentration for shoot formation

Summary

Introduction

Strawberry is an introduced horticultural crop in Sri Lanka and one of the best soft fruits in many parts of the world. Micropropagation of strawberry from runners has been reported and efficiently produces a large number of disease free plants. Inoculation of explants The MS (Murashige and Skoog) basal medium supplemented with different concentrations of BAP and IBA were used as the culture media (Table 1) to inoculate the sterilized explants.

Results

Conclusion