EFFECT OF BA AND TDZ IN VEGETATIVE MULTIPLICATION OF SHOOT TIPS AND NODES OF Buahinia purpurea L. In vitro

Abstract

This study aimed to develop a system for propagating of Camel's Foot Bauhinia purpurea L. by tissue culture technology. Seeds were germinated after removal the external coats in agar-solidified MS medium without growth regulators, In order to get fresh explants and sterilized for use in the following study stages. After 4 weeks of germination and a suitable high of seedlings explants from growing tips and nodes were excised and cultured in MS medium supplemented with different concentrations of TDZ and BA (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/l) of each during initiation and replication stages in order to get a highest number of multiplied new shoots, Cultured growing tips showed that 1.5 mg/l of BA produced the shoots numerous average 2.66 using 2.5 and 3.0 mg/l of TDZ also have largest shoots average 3.10. On the other hand, nodes cultured on 3.0 mg/l BA have highest shoots average 6.44 at a time TDZ with concentration of 2.0 mg/l also have highest shoots number 7.44. Results showed in multiplication stage for growing tips and nodes which we have from initiation stage cultured in solid MS medium with same concentration of BA and TDZ have a good responses. So BA with 1.5 mg/l of concentration has largest shoots numbers 7.55 when 2.5 mg/l BA was the best in case of shoots length with 33.94 cm. But culturing nodes showed that 3.0 mg/l of BA was the best and have largest averages of shoot numbers 8.22 when in the case of shoots numbers characteristics we found that the concentration of 2.0 mg/l BA was the best with record of 21.49 cm. The results proved that TDZ in 2.0 mg/l have largest average shoots numbers 9.66.