Abstract
Abstract The aim of this study was to establish plant regeneration from leaf explants of Sedum tosaense Makino, which is globally rare and endangered species. The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for callus induction. Callus induction was showed the highest (100%) on MS medium containing 2.0 mg ․L -1 BA and 1.0 mg․L -1 NAA. The highest number of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg․L -1 BA and 1.0 mg ․L -1 NAA for 5 weeks. The ax illary bud were cultured on the MS media supplemented with com- bination of BA and NAA for in vitro propagation. The highest number of adventitious shoot (7.9 per explants) formed at 1.0 mg․L -1 NAA and 2.0 mg․L -1 BA. For rooting, MS medium supplemented with or without 2.0 g ․L -1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g ․L -1 activated charcoal, on which 85.7 (No. of root), 4.6 cm (length of root). 1,200 ppm CO
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