Abstract
Objective To evaluate the effect of aurora kinase A （AURKA） silencing on the viability of prostate cancer cells. Methods AURKA expression in DUI45 cells was inhibited by two specific small hairpin RNAs （shRNAs） from eight shRNAs, and the viability of DU145 cells was documented by methyl thiazol tetrazolium （MTT） assay. Results Inhibition of AURKA by two shRNAs markedly reduced the viability of prostate cancer cells by 40. 5% （ P 〈 0. 01 ） and 61.3% （ P 〈 0. 01 ）, and increase apoptotic ratio by 9.23 % （ P 〈 0. 05 ） and 15.45% （ P 〈 0. 05 ）, respectively. Conclusion AURKA knockdown of AR-DU145 cells can be achieved by effective shRNA sequences through an apoptosis-promoting approach. Key words: Prostate cancer; Aurora kinase A; Androgen receptor
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